Subject: FDA Mechanisms of SARS-CoV-2 Cellular Entry Internship

Description:
A research opportunity is available in the Office of Pharmaceutical
Quality/Office of Biotechnology Products (OBP), Center for Drug Evaluation
and Research (CDER), Food and Drug Administration (FDA) located in Silver
Spring, Maryland.

This project explores the mechanisms of SARS-CoV-2 spike protein-mediated
cell fusion using engineered non-infectious virus-like particles (VLP),
focusing on the effect of different cytokines in human lung-derived
epithelial cell lines, primary human lung epithelial cells, and/or human
macrophages. A variety of similarly generated VLP, pseudotyped with
alternative virus envelope glycoproteins will be studied as well. The project
will also investigate VLP-induced cytokine secretion in various cell types
and evaluate potential virus entry inhibitors.

Under the guidance of the mentor, the participant will learn laboratory and
research techniques to perform experiments utilizing a variety of methods
such as PCR, DNA purification, electrophoresis, Western blot analysis, flow
cytometry, fluorescent microscopy, cell culture, etc. In addition, the
participant will gain understanding of the mechanisms of virus entry.

Contact Person: ORISE
Contact Email: [log in to unmask]
Website URL:
https://www.zintellect.com/Opportunity/Details/FDA-CDER-2020-0591

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