My name is Gary Brown and I am with MRIGlobal, formerly known as Midwest Research Institute. The reason I am contacting you is for some help. We currently have an assistant level position in virology for a recent or new graduate. This position will be located in our Rockville, MD lab. Research Duties include: • Performs assays and experiments in molecular biology to include nucleic acid extraction, PCR, real time PCR, cloning, plasmid preparation, mutagenesis, gel electrophoresis, and sequence analysis. • Performs transfections of eukaryotic or prokaryotic cells, confirmation of plasmid expression to include immunofluorescence assays, gel electrophoresis and western blot analysis. • Performs in vitro transcription, protein production and purification, co-immunoprecipitation. • Maintains cell cultures needed for specific experiments. • ELISA assays and viral plaque assays • Identifies and solves common problems relating to laboratory methodology, instrumentation and procedure under the direction of their supervisor. • Participates in development of assays used in the laboratory, and training others on equipment and assays. • Maintains adequate inventory of supplies as required by the department. • Maintains a laboratory notebook and meets with supervisor to review data. • Enters data into database adhering to specifications and support online protocols and amendments. General Lab Duties include: • General lab organization, maintenance and cleaning. • Maintain cell line, plasmid, freezer, and reagent inventories. • Prepare packages for shipment; maintain supplies, common lab stocks, and lab equipment. Any help you could provide will be appreciated. Thank you for your time Sincerely, Brown, Gary <[log in to unmask]> 425 Volker Blvd | Kansas City, MO 64110 (816) 360-1905 direct | (816) 753-2304 fax