Research Assistant I - HJF is seeking a Research Assistant to support the US Military HIV Research Program (MHRP)at Walter Reed Army Institute of Research(WRAIR) located in Silver Spring, MD. Incumbent will work in the Specimen Processing Laboratory in support of clinical trials, diagnostic efforts and the Infectious Disease Clinical Research Program (IDCRP) goals and objectives. The individual will provide technical and basic laboratory support to senior technical and scientific staff. The studies will include assessment of the functional status of the immune system and its relationship to cellular phenotypes and viral loads in humans and animals receiving vaccines as well as participating in other laboratory investigations of virologic and immunological aspects of HIV and other infectious diseases. HJF provides scientific, technical and programmatic support services to MHRP. RESPONSIBILITIES: 1. Isolation and storage of peripheral mononuclear blood cells. 2. Preparation of primary tissues for single cell suspensions. 3. Preparation of cells from peripheral blood for immunophenotyping. 4. Passage of cell lines in continuous culture. 5. Preparation of cell culture media and reagents. 6. Separation and storage of biological fluids. 7. Preparation of buffers, reagents and stocks. 8. Perform data entry for research projects. 9. Maintain databases and equipment. 10. Maintain clear and accurate laboratory records and inventories. 11. Maintain supplies in the laboratory and keep lab areas clean. 12. Provides other support, as needed, to infectious disease diagnostic and research programs. Required Knowledge, Skills, and Abilities: Excellent laboratory and technical skills; ability to work comfortably in a biosafety level two plus (p-2 plus) laboratory with HIV, HCV, HTLV and other infectious pathogens; ability to work independently; ability to keep good records; good communication and analytical skills. Minimum Education/Training Requirements: Bacheloršs degree in related scientific field Minimum Experience: Research project experience during undergraduate training or job experience in cell biology techniques (minimum experience 0-2 years). Work Environment: Henry M. Jackson Foundation in Rockville, MD. Will handle biohazardous materials including infectious agents such as HIV and is expected to practice safe handling of hazardous chemicals. Any qualifications to be considered as equivalents, in lieu of stated minimums, require the prior approval of the Vice President of Human Resources Contact: J. Connor Eggleston MHRP BioRepository and Specimen Processing Laboratory Manager Henry M. Jackson Foundation 13 Taft Court, Room 13 Rockville, MD 20850 301-251-8345 [log in to unmask]